Western Blot:Article Title: An essential checkpoint for TLR9 signaling is release from Unc93b1 in endosomes
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10; Roche), mouse anti-FLAG as purified antibody or matrix (M2; Sigma-Aldrich), anti-mLamp-1 (goat polyclonal, AF4320; R&D Systems), anti-calnexin (rabbit polyclonal; Enzo Life Sciences, ADI-SPA-860-F), anti-Myd88 (AF3109, R&D Systems), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22; eBioscience), purified anti-CD16/32 Fc Block (2.4G2), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10; Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Article Title: Unc93b1 recruits Syntenin-1 to dampen TLR7 signaling and prevent autoimmunity
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-mLamp-1 (AF4320, R&D Systems), anti-Calnexin (ADI-SPA-860, Enzo Life Sciences), anti-Gapdh (GT239, GeneTex), anti-Myd88 (AF3109, R&D Systems), anti-IRAK2 (Cell Signaling), anti-Phospho-p38 (Cell Signaling), anti-p38 (Cell Signaling), anti-Phospho-SAPK/JNK (81E11, Cell Signaling), anti-SAPK/JNK (56G8, Cell Siganling), anti-Phospho-p44/42 (ERK1/2) (D13.14.4E, Cell Signaling), anti-p44/42 (ERK1/2) (137F5, Cell Siganling), anti-IκBα (Cell Signaling), anti-Syntenin-1 (2C12, Novusbio), anti-Unc93b1 (PA5–20510, Thermo Scientific), anti-ubiquitin (P4D1, Santa Cruz), anti-K63-linked ubiquitin (human polyclonal, kind gift from Michael Rape), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-human IRDye 680RD (Licor), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. Antibodies for immunofluorescence were: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat IgG-AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch).
Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma), anti-Unc93b1 (1:1000, PA5–20510, Thermo Scientific), anti-GAPDH (1:1000, MA5-15738, Invitrogen), anti-mLamp-1 (1:1000, AF4320, R&D Systems), anti-Phospho-p38 (1:1000, D3F9, 4511T, Cell Signaling), anti-p38 (1:1000, D13E1, 8690T, Cell Signaling), anti-Phospho-p44/42 (ERK1/2) (1:1000, 197G2, 4377T, Cell Signaling), anti-p44/42 (ERK1/2) (1:1000, 137F5, Cell Signaling), anti-IκBα (1:1000, L35A5, 4814, Cell Signaling), anti-Borcs5 (1:500, 17169-1-AP, Proteintech), anti-Borcs7 (1:500, PAB23142, Abnova), anti-Arl8b (1:500, 13049-1-AP, Proteintech), anti-TLR7 (1:500, MAB7156, R&D systems), anti-EEA1 (1:1000, clone C45B10, 3288S, Cell Signaling), anti-LC3b (1:1000,clone D11,3868,Cell Signaling), anti-Rab7 (1:1000, D95F2, 9367, Cell Signaling), anti-Rab5 (1:1000, C8B1, 3547, Cell Signaling). .. Secondary antibodies: AlexaFluor 680 anti-mouse IgG (A21057, Invitrogen), AlexaFluor 680 anti-rabbit IgG (A21076, Invitrogen), anti-Rabbit HRP (111-035-144, Jackson ImmunoResearch), anti-Mouse HRP (115-035-166, Jackson ImmunoResearch), anti-Rat HRP (112-035-062, Jackson ImmunoResearch).
Article Title: Unc93b1 recruits Syntenin-1 to dampen TLR7 signaling and prevent autoimmunity
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-mLamp-1 (AF4320, R&D Systems), anti-Calnexin (ADI-SPA-860, Enzo Life Sciences), anti-Gapdh (GT239, GeneTex), anti-Myd88 (AF3109, R&D Systems), anti-IRAK2 (Cell Signaling), anti-Phospho-p38 (Cell Signaling), anti-p38 (Cell Signaling), anti-Phospho-SAPK/JNK (81E11, Cell Signaling), anti-SAPK/JNK (56G8, Cell Siganling), anti-Phospho-p44/42 (ERK1/2) (D13.14.4E, Cell Signaling), anti-p44/42 (ERK1/2) (137F5, Cell Siganling), anti-IκBα (Cell Signaling), anti-Syntenin-1 (2C12, Novusbio), anti-Unc93b1 (PA5-20510, Thermo Scientific), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. Antibodies for immunofluorescence were: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat IgG-AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch).
Article Title: Release from Unc93b1 reinforces the compartmentalized activation of select TLRs
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10, Roche), mouse anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-V5 (rabbit polyclonal, AB3792, Millipore), anti-mLamp-1 (goat polyclonal, AF4320, R&D Systems), anti-Calnexin (rabbit polyclonal, ADI-SPA-860-F, Enzo Life Sciences), rabbit anti-Rab5 (C8B1, Cell Signaling), anti-EEA1 (rabbit polyclonal, 2411S, Cell Signaling), mouse anti-GM130 (610822, BD), anti-TLR9 (B33A4, kind gift from Kensuke Miyake), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22, eBioscience), CD19 (6D5, BioLegend), purified anti-CD16/32 Fc Block (2.4G2, BD), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-rat IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), donkey anti-rat AlexaFluor488 (Jackson Immunoresearch), donkey anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Flow Cytometry:Article Title: An essential checkpoint for TLR9 signaling is release from Unc93b1 in endosomes
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10; Roche), mouse anti-FLAG as purified antibody or matrix (M2; Sigma-Aldrich), anti-mLamp-1 (goat polyclonal, AF4320; R&D Systems), anti-calnexin (rabbit polyclonal; Enzo Life Sciences, ADI-SPA-860-F), anti-Myd88 (AF3109, R&D Systems), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22; eBioscience), purified anti-CD16/32 Fc Block (2.4G2), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10; Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Article Title: Release from Unc93b1 reinforces the compartmentalized activation of select TLRs
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10, Roche), mouse anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-V5 (rabbit polyclonal, AB3792, Millipore), anti-mLamp-1 (goat polyclonal, AF4320, R&D Systems), anti-Calnexin (rabbit polyclonal, ADI-SPA-860-F, Enzo Life Sciences), rabbit anti-Rab5 (C8B1, Cell Signaling), anti-EEA1 (rabbit polyclonal, 2411S, Cell Signaling), mouse anti-GM130 (610822, BD), anti-TLR9 (B33A4, kind gift from Kensuke Miyake), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22, eBioscience), CD19 (6D5, BioLegend), purified anti-CD16/32 Fc Block (2.4G2, BD), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-rat IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), donkey anti-rat AlexaFluor488 (Jackson Immunoresearch), donkey anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Purification:Article Title: An essential checkpoint for TLR9 signaling is release from Unc93b1 in endosomes
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10; Roche), mouse anti-FLAG as purified antibody or matrix (M2; Sigma-Aldrich), anti-mLamp-1 (goat polyclonal, AF4320; R&D Systems), anti-calnexin (rabbit polyclonal; Enzo Life Sciences, ADI-SPA-860-F), anti-Myd88 (AF3109, R&D Systems), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22; eBioscience), purified anti-CD16/32 Fc Block (2.4G2), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10; Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Article Title: Unc93b1 recruits Syntenin-1 to dampen TLR7 signaling and prevent autoimmunity
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-mLamp-1 (AF4320, R&D Systems), anti-Calnexin (ADI-SPA-860, Enzo Life Sciences), anti-Gapdh (GT239, GeneTex), anti-Myd88 (AF3109, R&D Systems), anti-IRAK2 (Cell Signaling), anti-Phospho-p38 (Cell Signaling), anti-p38 (Cell Signaling), anti-Phospho-SAPK/JNK (81E11, Cell Signaling), anti-SAPK/JNK (56G8, Cell Siganling), anti-Phospho-p44/42 (ERK1/2) (D13.14.4E, Cell Signaling), anti-p44/42 (ERK1/2) (137F5, Cell Siganling), anti-IκBα (Cell Signaling), anti-Syntenin-1 (2C12, Novusbio), anti-Unc93b1 (PA5–20510, Thermo Scientific), anti-ubiquitin (P4D1, Santa Cruz), anti-K63-linked ubiquitin (human polyclonal, kind gift from Michael Rape), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-human IRDye 680RD (Licor), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. Antibodies for immunofluorescence were: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat IgG-AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch).
Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma), anti-Unc93b1 (1:1000, PA5–20510, Thermo Scientific), anti-GAPDH (1:1000, MA5-15738, Invitrogen), anti-mLamp-1 (1:1000, AF4320, R&D Systems), anti-Phospho-p38 (1:1000, D3F9, 4511T, Cell Signaling), anti-p38 (1:1000, D13E1, 8690T, Cell Signaling), anti-Phospho-p44/42 (ERK1/2) (1:1000, 197G2, 4377T, Cell Signaling), anti-p44/42 (ERK1/2) (1:1000, 137F5, Cell Signaling), anti-IκBα (1:1000, L35A5, 4814, Cell Signaling), anti-Borcs5 (1:500, 17169-1-AP, Proteintech), anti-Borcs7 (1:500, PAB23142, Abnova), anti-Arl8b (1:500, 13049-1-AP, Proteintech), anti-TLR7 (1:500, MAB7156, R&D systems), anti-EEA1 (1:1000, clone C45B10, 3288S, Cell Signaling), anti-LC3b (1:1000,clone D11,3868,Cell Signaling), anti-Rab7 (1:1000, D95F2, 9367, Cell Signaling), anti-Rab5 (1:1000, C8B1, 3547, Cell Signaling). .. Secondary antibodies: AlexaFluor 680 anti-mouse IgG (A21057, Invitrogen), AlexaFluor 680 anti-rabbit IgG (A21076, Invitrogen), anti-Rabbit HRP (111-035-144, Jackson ImmunoResearch), anti-Mouse HRP (115-035-166, Jackson ImmunoResearch), anti-Rat HRP (112-035-062, Jackson ImmunoResearch).
Article Title: Unc93b1 recruits Syntenin-1 to dampen TLR7 signaling and prevent autoimmunity
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-mLamp-1 (AF4320, R&D Systems), anti-Calnexin (ADI-SPA-860, Enzo Life Sciences), anti-Gapdh (GT239, GeneTex), anti-Myd88 (AF3109, R&D Systems), anti-IRAK2 (Cell Signaling), anti-Phospho-p38 (Cell Signaling), anti-p38 (Cell Signaling), anti-Phospho-SAPK/JNK (81E11, Cell Signaling), anti-SAPK/JNK (56G8, Cell Siganling), anti-Phospho-p44/42 (ERK1/2) (D13.14.4E, Cell Signaling), anti-p44/42 (ERK1/2) (137F5, Cell Siganling), anti-IκBα (Cell Signaling), anti-Syntenin-1 (2C12, Novusbio), anti-Unc93b1 (PA5-20510, Thermo Scientific), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. Antibodies for immunofluorescence were: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat IgG-AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch).
Article Title: Release from Unc93b1 reinforces the compartmentalized activation of select TLRs
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10, Roche), mouse anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-V5 (rabbit polyclonal, AB3792, Millipore), anti-mLamp-1 (goat polyclonal, AF4320, R&D Systems), anti-Calnexin (rabbit polyclonal, ADI-SPA-860-F, Enzo Life Sciences), rabbit anti-Rab5 (C8B1, Cell Signaling), anti-EEA1 (rabbit polyclonal, 2411S, Cell Signaling), mouse anti-GM130 (610822, BD), anti-TLR9 (B33A4, kind gift from Kensuke Miyake), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22, eBioscience), CD19 (6D5, BioLegend), purified anti-CD16/32 Fc Block (2.4G2, BD), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-rat IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), donkey anti-rat AlexaFluor488 (Jackson Immunoresearch), donkey anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Blocking Assay:Article Title: An essential checkpoint for TLR9 signaling is release from Unc93b1 in endosomes
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10; Roche), mouse anti-FLAG as purified antibody or matrix (M2; Sigma-Aldrich), anti-mLamp-1 (goat polyclonal, AF4320; R&D Systems), anti-calnexin (rabbit polyclonal; Enzo Life Sciences, ADI-SPA-860-F), anti-Myd88 (AF3109, R&D Systems), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22; eBioscience), purified anti-CD16/32 Fc Block (2.4G2), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10; Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Article Title: Release from Unc93b1 reinforces the compartmentalized activation of select TLRs
Article Snippet: .. The following antibodies were used for immunoblots, immunoprecipitations, or flow cytometry: rat anti-HA as purified antibody or matrix (3F10, Roche), mouse anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-V5 (rabbit polyclonal, AB3792, Millipore), anti-mLamp-1 (goat polyclonal, AF4320, R&D Systems), anti-Calnexin (rabbit polyclonal, ADI-SPA-860-F, Enzo Life Sciences), rabbit anti-Rab5 (C8B1, Cell Signaling), anti-EEA1 (rabbit polyclonal, 2411S, Cell Signaling), mouse anti-GM130 (610822, BD), anti-TLR9 (B33A4, kind gift from Kensuke Miyake), anti-Gapdh (GT239, GeneTex), anti-TNFα-APC (MP6-XT22, eBioscience), CD19 (6D5, BioLegend), purified anti-CD16/32 Fc Block (2.4G2, BD), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-rat IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. For immunofluorescence: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), donkey anti-rat AlexaFluor488 (Jackson Immunoresearch), donkey anti-rabbit AlexaFluor647 (Jackson Immunoresearch).
Ubiquitin Proteomics:Article Title: Unc93b1 recruits Syntenin-1 to dampen TLR7 signaling and prevent autoimmunity
Article Snippet: .. The following antibodies were used for immunoblots and immunoprecipitations: anti-HA as purified antibody or matrix (3F10, Roche), anti-FLAG as purified antibody or matrix (M2, Sigma-Aldrich), anti-mLamp-1 (AF4320, R&D Systems), anti-Calnexin (ADI-SPA-860, Enzo Life Sciences), anti-Gapdh (GT239, GeneTex), anti-Myd88 (AF3109, R&D Systems), anti-IRAK2 (Cell Signaling), anti-Phospho-p38 (Cell Signaling), anti-p38 (Cell Signaling), anti-Phospho-SAPK/JNK (81E11, Cell Signaling), anti-SAPK/JNK (56G8, Cell Siganling), anti-Phospho-p44/42 (ERK1/2) (D13.14.4E, Cell Signaling), anti-p44/42 (ERK1/2) (137F5, Cell Siganling), anti-IκBα (Cell Signaling), anti-Syntenin-1 (2C12, Novusbio), anti-Unc93b1 (PA5–20510, Thermo Scientific), anti-ubiquitin (P4D1, Santa Cruz), anti-K63-linked ubiquitin (human polyclonal, kind gift from Michael Rape), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), goat anti-mouse IgG-AlexaFluor680 (Invitrogen), rabbit anti-goat IgG-AlexaFluor680 (Invitrogen), goat anti-human IRDye 680RD (Licor), goat anti-mouse IRDye 800CW (Licor), donkey anti-rabbit IRDye 680RD (Licor), goat anti-rat IRDye 800CW (Licor). .. Antibodies for immunofluorescence were: rat anti-HA (3F10, Roche), rabbit anti-Lamp1 (ab24170, Abcam), goat anti-rat IgG-AlexaFluor488 (Jackson Immunoresearch), goat anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch).
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